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Introduction To N-Hydroxy Succinimide

N-Hydroxy Succinimide is a white powder or colorless crystal, easily soluble in many kinds of solvents, resolved in succinic acid and ammonia with water. N-Hydroxy succinimide functions by stabilizing the protein-EDC intermediate, thus allowing for a more efficient crosslinking reaction.
N-hydroxysuccinimide (NHS) esters have been used for gas-phase conjugation reactions with peptides at nucleophilic sites, such as primary aminesor guanidines, by forming amide bonds through a nucleophilic attack on the carbonyl carbon. The carboxylate has recently been found to also be a reactive nucleophile capable of initiating a similar nucleophilic attack to form a labile anhydride bond.
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